本帖最后由 comet 于 2026-1-16 21:32 编辑
首先大白点是刺激隐核虫（Cryptocaryon irritans），相关文献不是一搜就有吗。
Cryptocaryon irritans(Ciliophora): acquired protective immunity in the thick-lipped mullet,Chelon labrosus
Acquired protection to Cryptocaryon irritans has been demonstrated for the first time, using the grey mullet,Chelon labrosus, as an experimental host. Fish, immunized by controlled infections, established immunity against challenge infections withC. irritans, the degree of protection correlating with both intensity and exposure levels, with relatively few fish developing full protection. Protection lasted for six months in the absence of re-exposure to the parasite. There was no evidence of cross-protection in mullet against infection with the closely related fish parasite,Ichthyophthirius multifiliis.
首次使用鲻鱼（Chelon labrosus）作为实验宿主，证明了其对白点虫（Cryptocaryon irritans）的获得性保护。通过控制感染对鱼类进行免疫，使其对白点虫的攻击性感染产生免疫力，保护程度与感染强度和暴露水平相关，相对较少的鱼能获得完全保护。在未再次暴露于寄生虫的情况下，保护效果持续六个月。没有证据表明鲻鱼对与其密切相关的鱼类寄生虫——多子小瓜虫（Ichthyophthirius multifiliis）的感染具有交叉保护作用。
小白点 大陆正式称呼为淀粉卵涡鞭虫（Amyloodinium ocellatum），台湾鱼友叫卵圆鞭毛虫，相关文献
Acquired immunity to amyloodiniosis is associated with an antibody response - PubMed
The dinoflagellate Amyloodinium ocellatum, which causes amyloodiniosis or 'marine velvet disease', is one of the most serious ectoparasitic diseases plaguing warmwater marine fish culture worldwide. We report that tomato clownfish Amphiprion frenatus develop strong immunity to Amyloodinium ocellatum infection following repeated nonlethal challenges and that specific antibodies are associated with this response. Reaction of immune fish antisera against dinospore and trophont-derived antigens in Western blots indicated both shared and stage-specific antibody-antigen reactions. A mannan-binding-protein affinity column was used to isolate IgM-like antibody from A. frenatus serum. The reduced Ig consisted of one 70 kD heavy chain and one 32 kD light chain with an estimated molecular weight of 816 kD for the native molecule. Immunoglobulin (Ig) isolated from immune but not non-immune fish serum significantly inhibited parasite infectivity in vitro. An enzyme-linked immunosorbent assay (ELISA) was developed using polyclonal rabbit antibody produced against affinity-purified A. frenatus Ig. Anti-Amyloodinium serum antibody was not always detectable in immune fish, although serum antibody titers in immune fish increased after repeated exposure to the parasite. These results suggest that there may be a localized antibody response in skin/gill epithelial tissue, although antibody was rarely detected in skin mucus.
对淀粉样变性病的获得性免疫与抗体反应相关
摘要： 引起淀粉样变性病或海洋丝绒病的甲藻淀粉卵涡鞭虫是世界范围内困扰温水海水鱼类养殖的最严重的体外寄生虫病之一。我们报告，番茄小丑鱼在经历重复的非致死性攻击后，对淀粉卵涡鞭虫感染产生了强烈的免疫力，并且特异性抗体与此反应相关。免疫鱼抗血清与游动孢子和滋养体来源抗原在免疫印迹中的反应表明存在共享的和阶段特异性的抗体-抗原反应。使用甘露聚糖结合蛋白亲和层析柱从番茄小丑鱼血清中分离出了 IgM 样抗体。还原后的免疫球蛋白由一条 70 kD 的重链和一条 32 kD 的轻链组成，推测天然分子的分子量约为 816 kD。从免疫鱼（而非非免疫鱼）血清中分离的免疫球蛋白在体外显著抑制了寄生虫的感染性。使用针对亲和纯化的番茄小丑鱼免疫球蛋白产生的多克隆兔抗体，建立了酶联免疫吸附测定法。在免疫鱼中并非总能检测到抗淀粉卵涡鞭虫血清抗体，尽管免疫鱼在反复接触寄生虫后血清抗体滴度有所增加。这些结果表明，可能在皮肤/鳃上皮组织中存在局部的抗体反应，尽管在皮肤粘液中很少检测到抗体。
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